Tag

Part:BBa_K1486026:Design

Designed by: EPFL iGem team 2014   Group: iGEM14_EPF_Lausanne   (2014-09-26)

sfGFP + Kanamycin resistance for yeast


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2475
    Illegal PstI site found at 1239
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2475
    Illegal PstI site found at 1239
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2475
    Illegal BglII site found at 1029
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2475
    Illegal PstI site found at 1239
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2475
    Illegal PstI site found at 1239
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The flanking sequences are sequences homologous to the S.Cerevisiae genome: the last 50 coding bases of the Pbs2 gene and the 50 first non coding bases at its end, which target the insertion of the transformation vector.

Source

It was extracted by PCR from plasmids we ordered to addgene:Plasmid 44901 pFA6a-link-yoSuperfolderGFP-Kan.

References

Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors for S. cerevisiae. Lee et al (PLoS One. 2013 Jul 2;8(7):e67902. doi: 10.1371/journal.pone.0067902. Print 2013. PubMed)